In order to get the rodent dispute in-frame, Rowley explained that he had to lay flat on the subway platform, working five late nights to get the newly award-winning shot.
The museum stated on its website that Rowley took serious effort to get the ideal click.
The photo was selected from a shortlist of 25 images that were culled from 48,000 entries.
"I will continue other urban wildlife photography projects in Britain and around the world over the next year", he told me.
Director of the Natural History Museum, Sir Michael Dixon, says: "Sam's image provides a fascinating glimpse into how wildlife functions in a human-dominated environment".
"One moment stands out in particular".
Four other photographs were highly commended. Sam Rowley has said that his picture shows people unexpected drama in familiar urban environment, reports PetaPixel. His patience paid off when two mice battled over a leftover crumb, the encounter lasting only a split-second before one ran off a victor.
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Another, "Losing the fight", depicts an orangutan wearing boxing gloves and shorts - a critique of the degrading performances the animals are forced to partake at Safari World in Bangkok and across wider Asia.
"I'm so pleased to win this award", Rowley said in the museum's news release. Twice a day, hundreds of people still pay to watch orangutans fight, dance and play the drums.
He added: "This image reminds us that while we may wander past it every day, humans are inherently intertwined with the nature that is on our doorstep".
Matching outfits by Michel Zoghzoghi, Lebanon - Michel was in the Pantanal, Brazil photographing jaguars.
"Elias Mugambi is a ranger at Lewa Wildlife Conservancy in northern Kenya". The compelling photograph shows two jaguars, a mother and cub, carrying an anaconda out of the river.
The 56th WPY competition is now being judged by a panel of experts, and its grand prize winners will be revealed in October.
"Spot the reindeer" by Spanish photographer Francis De Andres takes the viewer deep into the freezing wilderness of the Norwegian archipelago, Svalbard.