Most cases of the new coronavirus are mild, but the respiratory illness can be deadly in some people.
Seven cases of novel coronavirus have been confirmed in Canada. Three have been found in Ontario, while four were diagnosed in British Columbia. There were 130 Canadians and 58 accompanying family members aboard the flight.
More Canadians have arrived home after being evacuated from China.
Although consular officials were in contact with Canadians in China who have requested assistance, this is the last planned flight from Wuhan, the government said in a statement.
The flight is headed first to Hong Kong where it will have to wait before going into Wuhan.
The plane is bringing back the last group of Canadians who want to be repatriated, said Champagne, who is travelling with Prime Minister Justin Trudeau in Africa and the Middle East.
There are 236 Canadians waiting to board the plane from a city that has been under quarantine for weeks as Chinese authorities try to contain the virus's spread, Canadian officials said Sunday.
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Including those on this flight and the first evacuation plane, which left Wuhan last week, almost 400 people are under a two-week quarantine at the Canadian Forces Base in Trenton, Ont.
The plane touched down at Canadian Forces Base Trenton just after 6 a.m. local time on Tuesday morning.
The passengers from the first two flights have already begun their 14-day quarantine at a hotel-like accommodation on the base where they are being monitored for any signs of infection.
The Public Health Agency of Canada says the 25 flight crew members who accompanied the returning treavellers on the flight from Wuhan to Vancouver to CFB Trenton will be released early. "None of the passengers exhibited symptoms of the novel coronavirus upon arrival".
The risk to Canadians from coronavirus "continues to be low", according to Canada's Chief Public Health Officer, Dr. Theresa Tam.
In a press release, the federal government advised Canadians in China outside of Hubei province, whose presence there is "non-essential", to consider leaving by commercial means.
CNN reported 43,090 confirmed cases worldwide. Those patients have been taken to Japanese hospitals for treatment and monitoring. Each room has its own sink but washrooms are shared and will need to be cleaned frequently to make sure quarantine protocols are followed, Tam said. "I wanted to make sure we're all there for the families, for the people who are there".