"He knows what happened, I know what happened. And there's only one of us telling the truth". The image caused a scandal for the royal family when it first surfaced in 2015, along with an array of serious allegations of sexual misconduct.
Ms Giuffre, who was known as Virginia Roberts at the time, has said she was trafficked by convicted sex offender Jeffrey Epstein and had sex three times with Prince Andrew starting in 2001, including once in London.
Virginia Giuffre spoke to BBC's Panorama about claims she was forced to have sex with the royal when she was 17.
Prince Andrew's recent interview cast doubt over his side of the story about his friendship with paedophile Jeffrey Epstein, which has made Charles' plan more of a reality, according to the Daily Star. She says now that Andrew is out of the picture, she doesn't see him undertaking royal duties again.
"And there's only one of us telling the truth", Giuffre told the BBC in an interview to air Monday.
Andrew's interview was criticised by the public and global media, with one editor labelling it "plane crashing into an oil tanker, causing a tsunami, triggering a nuclear explosion-level bad".
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It will feature Virginia Giuffre's first United Kingdom television interview.
In a previous interview with Australia's 60 Minutes, Giuffre confirmed the photo of the two of them together was real and had been verified as an original.
"Prince Andrew has been given the worst-case scenario for him and his involvement with the family - that he may never be able to retrieve to the front line of duty", they added. "I know it's real".
71-year-old Charles, who is the rightful heir to the throne, has talked about cutting a number of working royals down to a bare minimum amid the Prince Andrew crisis, The Sun reports.
The Duke has always denied having slept with Ms Roberts, and told Emily Maitlis, the BBC Newsnight presenter, that he was with his daughter Princess Beatrice at a Pizza Express in Woking on the night in question.