Alexander-Arnold had been struggling with the problem before linking up with England and on Wednesday it was decided the right-back wouldn't recover in time to feature against the Czech Republic and Montenegro.
Alexander-Arnold has since played against Bayern Munich and Fulham, but was feeling his back issue again when he reported for global duty on Monday.
Liverpool defender Trent Alexander-Arnold has been forced to pull out of the England squad due to injury.
Ghana bus crash: Dozens killed after two buses collide in Bono East
The crash happened in Kitampo town, 430 km (270 miles) north of the capital Accra, at 0200 GMT on Friday morning, police say. Those with several degrees of injury have been transported to Jema, Kintampo and Techiman hospitals for treatment.
Former Michigan State basketball players defend Tom Izzo after outburst
One view: Izzo crossed the line of decorum even in a sport where coaching screaming is the norm. Cassius Winston ultimately restrained Izzo as he yelled at Henry.
Mail bomb suspect Cesar Sayoc pleads guilty
Prosecutors said the evidence against Sayoc included DNA that linked him to 10 of the devices and fingerprints on two of them. The bombs began turning up weeks before the hotly contested midterms, contributing to an already tense political environment.
Fabian Delph, John Stones, Luke Shaw and Ruben Loftus-Cheek have already pulled out, while Marcus Rashford is reportedly nursing an ankle complaint.
Gareth Southgate will now choose between Manchester City's Kyle Walker and Tottenham Hotspur's Kieran Trippier for tomorrow night's game against Czech Republic (7.45) at Wembley and Monday's trip to Montenegro.