The protest outside the London Stock Exchange building that stands in the shadow of St. Paul's Cathedral saw several glue themselves to the pavement in front of one of the glass entrance turnstiles.
Activists from the Christian Climate Action joined the demonstrations by holding signs stating "business as usual death" and "don't jail the canaries" whilst holding a prayer vigil.
Police used a ladder to remove them and they were later arrested.
On Thursday, they turned their attention to London's financial district, known as the City - home to more worldwide banks than any other and the global centre for foreign exchange trading.
More than 1,100 people have been arrested since campaigners from Extinction Rebellion first blocked traffic in the capital on 15 April.
The group issued a statement Wednesday that said, "We know we have disrupted your lives". We do not do this lightly.
The group is demanding the government declare a climate and ecological emergency, reduce greenhouse gas emissions to net zero by 2025 and create a citizen's assembly of members of the public to lead on decisions to address climate change.
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Police said 1088 arrests have been made since the main protests began last Monday.
Richard Barnard of Christian Climate Action - who is now on top of a train, said: "We would really rather not be here but the ravaging of God's creation calls us to do something, anything to stop this headlong rush into extinction on the back of the myth of financial progress and growth and business as usual".
A British Transport Police spokesman said: "We are now responding to a protest incident at Canary Wharf station where a @LondonDLR service is being obstructed".
"We have seen some action but is it enough to stop the huge emergency we are facing?"
The group also said the public should expect more action "very soon", adding there will be a "closing ceremony" at Speakers' Corner in Hyde Park on Thursday at 5pm.
"Specialist units trained in protester removal were immediately deployed and have now safely removed those obstructing the DLR service", the BTP said.
"The safety of our customers and staff is our number one priority and we're working closely with the police to manage the impact on London's transport network".
Demonstrators glue their hands to the London stock exchange during the Extinction Rebellion protest in London, Britain on April 25.