After assessing the situation with experts from a nearby zoo, they shot dead the nine-year-old lion that killed the man, as well as a lioness.
A Czech man who kept two lions as pets died from wounds inflicted by one of the felines, according to local police.
Prasek, 34, bought the lion in 2016 and the lioness past year. The lioness was reportedly pregnant. Pasek's body has now been recovered and sent for an autopsy to confirm the cause of death.
Mr Prasek, 34, kept the lion for breeding along with a lioness, which lived in a separate cage.
He was breeding them illegally and had been fined in the past because he did not have planning permission for the cages.
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Local authorities made several attempts to resolve the matter but it ended in a stalemate when he refused to allow authorities onto his property.
A lack of alternative facilities in the Czech Republic, or any evidence of animal cruelty, also meant the lions could not be forcibly removed.
Zdechov Mayor Tomas Kocourek told local media that Prasek would often walk his lions around the local area on a leash, prompting regular complaints from other local residents. The cyclist was hospitalized for his injuries.
A doctor who rushed to the scene of the accident was the first to "pronounce the man as dead", according to police, who added that the two animals never left the enclosure following the incident.