"We know who they are, we found them", Putin told a session at an economic forum in Vladivostok broadcast on state television.
British prosecutors last week identified two Russians who they said were operating under aliases - Alexander Petrov and Ruslan Boshirov - who they said had tried to murder the Skripals with a military-grade nerve agent in England.
Interpol released a "red notice" for the two men and the European Union put out an arrest warrant that might come into play if they ever leave Russian Federation again, but that also sounds unlikely.
Britain last week charged two alleged agents of Russia's military intelligence agency in absentia with the nerve agent poisoning of former Russian spy Sergei Skripal and his daughter Yulia in the English city of Salisbury.
Theresa May told parliament earlier this month that the attack "was nearly certainly also approved outside the GRU at a senior level of the Russian state". "We'll see in the near future".
Authorities believe the pair smeared the highly toxic chemical novichok on a door handle at the Wiltshire home of Sergei Skripal, leaving the former GRU officer and his daughter Yulia critically ill in March.
United Kingdom authorities have named the men as Alexander Petrov and Ruslan Boshirov, thought to be officers of Russia's military intelligence service, the GRU.
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Citing the GRU's reputation for strict discipline and hierarchy, May said of the attack on the Skripals, "It was nearly certainly also approved outside the GRU, at a senior level of the Russian state".
Prime Minister Theresa May said intelligence showed the poisonings were state-sponsored by Russian Federation. "I would like to call on them so that they can hear us today", he said Wednesday.
CCTV footage from early March shows the pair arriving at Heathrow Airport from Russian Federation, and also in the vicinity of Sergei Skripal's house on the day of the attack.
The statement by Putin marked an abrupt shift from Russia's earlier position on the poisoning case that has damaged relations between Moscow and the West.
"We hope they will turn up very soon and will tell everything themselves".
A couple who authorities said found the perfume bottle after it was discarded by the attackers fared worse: Charlie Rowley recovered after treatment in the hospital but his partner, Dawn Sturgess, 44, died on July 8.
Moscow also questioned the origins of the nerve agent involved in the attack, saying it was not proven that the substance was developed by Russian Federation and arguing that other countries, including Britain, had the capacity.