It was a historic moment for both the player and club.
Although it was widely known before the game that Bolt would make play a minor role in the match, the crowd of 9958 was around 2500 more than the Mariners' average home attendance last season.
Starting on the left wing, he fluffed his first touch, making a run on the inside only for the through ball to hit him on the heel.
The 32-year-old, who is on trial with the club, failed to find the target during the pre-season friendly.
The Olympic Champion has tried his luck at Borussia Dortmund and Norwegian outfit Strømsgodset in the past, but it never quite worked out.
The world-record holder in the 100- and 200-meters, Bolt said in a post-match interview that he did have some butterflies.
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Playing more centrally, his best touch came 10 minutes after coming on when he controlled a bobbling ball with his right foot, rode a challenge and laid it off with his left foot.
Six minutes after he came on, his team had scored a consolation goal before the match finished 6-1.
"I won't judge him on whether he can zip past somebody or get on the end of a header, we need to get him fit and give him time".
He wore 95 on his shirt, a reference to his 100-metre world record time of 9.58 set in Berlin nine years ago. "Four months I'll be playing like one of the guys", Bolt told Fox Sports.
The crowd of just under 10,000 gave him a loud ovation when he stepped onto the pitch, an uneasy smile on his often-beaming face. I was a bit nervous, but as soon as I got on the field it went away. "I've just got to put in the work, get up to speed and I'm looking forward to a great season".
'I think the first 15 was pretty good, the last five I could feel my back getting a little bit tight.
The Mariners are hoping to turn him into A-League material in time for the start of the 2018/19 season in late October but former Australian worldwide Robbie Slater, who was watching the game, said he still had plenty of work to do. He should also have more confidence to go and get the ball.