Isner will always be renowned at the All England Club for having won the longest grand slam match ever played, when he beat Nicolas Mahut on Court 18 in a first-round match lasting 11 hours and five minutes in 2010.
Anderson defeated Isner in the fifth set 7-6, 6-7, 6-7, 6-4, 26-24.
The second semifinal then was halted when the third set ended just past 11 p.m., because of a neighbourhood curfew.
The second semifinal of the day, pitting Rafael Nadal against Novak Djokovic, was easily the most highly-anticipated match of the tournament so far - and its start was delayed well over three hours as the city of London waited for either Isner or Anderson to blink.
Of the four grand slam tournaments, deciding set tiebreaks are only contested at the US Open when the score is at 6-all.
After covering a distance of 7,288.6 feet, Anderson lost his balance and fell while returning at 15-0 on Isner's serve, got back up on his feet and hit a hasty, left-handed forehand shot to keep himself in the game.
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If Steyn indeed plays, he will be looking to overhaul Shaun Pollock to become the highest Test wicket-taker for South Africa . If Chandimal misses out, pacer Suranga Lakmal could lead the team once again. "KG will take over that mantle [from Steyn]".
A total of 569 points were played, with Anderson winning 298 points and Isner winning 271.
If there were two tennis players prepared for this long-drawn combat, they were these two. Nadal saved two break points and when he finally held, the owner 17 major championships, two at Wimbledon, punched the air and yelled as if he'd won the match, not a solitary game.
"I think it is a completely new match", Kerber said. "At the end you don´t even feel that great out there, but at the same time I´m through to the final".
The 31-year-old is eyeing a 13th major after making the semi-finals of a Slam for the first time since finishing runner-up at the 2016 US Open. Another detriment of super-overtime is it's a real hardship on players scheduled to follow on the same court.
Even though Kevin Curren was South African by birth he became a naturalised American citizen in April 1985, before reaching the Wimbledon final later that year. "They're pretty antsy for us to get off the court".
By 14-all it seemed increasingly unlikely that either man would have the strength or mental sharpness to break serve, yet each had enough gas in the tank to hold, mustering that extra bit of power when it was needed.
While the Isner-Anderson marathon was taking place on Centre Court, there was another epic match being played under the radar over on Court 3.
Djokovic set himself up to take the first set with strong service games, facing no break points and broke in the seventh game before sealing it with a forehand victor.