The magnitude 6.1 natural disaster struck the city of Osaka and the surrounding area Monday about 8 a.m. local time.
An 80-year-old man and a young 9yo girl were killed in Japan, crushed by collapsing walls, public broadcaster NHK reported. The strongest shaking was in an area north of Osaka city, the agency said.
All trains and subways across Osaka city were halted, and the bullet train that travels through the region was halted as far as Fukuoka in southern Japan.
Local police said they could not confirm the reports.
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The Japan Meteorological Agency says a quake with preliminary magnitude of 5.9 struck Monday around 8 a.m. The initial strength of the quake was measured at 5.9.
While there were no reports of widespread damage, Japanese authorities are still assessing the impact, with PM Shinzo Abe emphasizing that the safety of residents is the government's top priority at the moment.
A powerful quake struck Osaka and neighboring prefectures on June 18, knocking out power in a widespread area and disrupting Shinkansen and local train services.
Pictures posted on Twitter showed signs at a train station that had fallen down and books thrown off shelves at a store.